Journal: Computational and Structural Biotechnology Journal

Article Title: IKKα Promotes the Progression and Metastasis of Non-Small Cell Lung Cancer Independently of its Subcellular Localization

doi: 10.1016/j.csbj.2019.02.003

Figure Lengend Snippet: Overexpression of IKKα both in the nucleus and cytoplasm of NSCLC cells enhances the growth of lung tumors in vivo. (A, B) Representative images showing tumors developed on the flanks of nude (A) and R2G2 (B) mice 11 days post-injection of the indicated type of H460 cells. Note the large size of tumors originated by inoculation of both C-and-N-H460-IKKα cells in both strains of immunodeficient mice. Each cell type was injected into six nude mice and four R2G2 mice. (C) Larger size of tumors that overexpress IKKα. Left: detail of tumors photographed on day 19-post-injection. Observe the larger size and the presence of blood vessels visible to naked eye in both the C-and-N-H460-IKKα tumors. Right: a representative graph of tumor growth curve of xenograft assay in nude mice is shown. Tumor growth was followed for 19 days and measured with an external caliper. Statistical significance was determined using the Bonferroni's multiple comparison test (* P < .05). (D-F) Immunochemistry with an anti-IKKα-specific antibody shows enhanced staining in the cytoplasm of the C-H460-IKKα tumors (E) and in the nucleus of the N-H460-IKKα ones (F). Lower expression of IKKα is detected in tumors derived from the Control-H460 cells (D). (G) Representative image showing the appearance of the ADCs derived from the H460 cells of the three genotypes (a C-H460-IKKα tumor is shown). (H-K) Representative example of areas of necrosis (asterisk in H); anisocaryosis (yellow triangles in I); multinucleated giant cell (yellow arrow in J), and aberrant mitosis (black arrows in K) in C-H460-IKKα tumors. Scale bars: 50μm (D-F); 250 μm (G-H); 40 μm (I-K). (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: Tumors were induced in immunodeficient mice (Rag2/IL2RG Double Knockout (R2G2), generously donated by Envigo, Spain; and in Hsd-Athymic Nude (Harlan, Barcelona, Spain).

Techniques: Over Expression, In Vivo, Injection, Xenograft Assay, Comparison, Staining, Expressing, Derivative Assay, Control

Journal: Computational and Structural Biotechnology Journal

Article Title: IKKα Promotes the Progression and Metastasis of Non-Small Cell Lung Cancer Independently of its Subcellular Localization

doi: 10.1016/j.csbj.2019.02.003

Figure Lengend Snippet: Increased metastatic capacity of the C-and-N-H460-IKKα adenocarcinomas. (A-K) Macroscopic and histological analysis of the lungs (A-I) and liver (J, K) of mice injected subcutaneously with H460 cells of the three genotypes. Observe the less frequent development of lung metastasis (black arrow) in immunodeficient mice injected with the Control-H460 cells (A) compared to the abundant foci of lung metastasis (whitish formations, black arrows in D, G) in both C-and-N-IKKα lungs. (B,C) Representative images showing the histology of lungs of mice injected with the Control-H460 cells; observe the scarce number and low size of the tumor metastasis (m). (E, F, H, I) Abundant and large metastasis (m) were detected in the lungs of mice inoculated with C-H460-IKKα (E, F) or N-H460-IKKα (H, I) cells. One metastasis was found in the liver of a mice injected with N-H460-IKKα cells (J, K). (L-N) Immunostaining showing increased expression of IKKα in the cytoplasms of the metastasis of mice injected with C-H460-IKKα cells (M) and in the nuclei of metastasis of mice inoculated with N-H460-IKKα cells (N). Expression of IKKα was also detected in the metastasis of mice receiving Control-H460 cells (L). (O-Q) Immunostaining showing the nuclear expression of TTF1, in the metastasis of the lungs of mice injected with the H460 cells of the three genotypes. lu: lung tissue; m: metastasis; lv: liver tissue. Scale bars: 350 μm (B, C, E, F, H, I, K); 120 μm (L-N); 100 μm (O-Q).

Article Snippet: Tumors were induced in immunodeficient mice (Rag2/IL2RG Double Knockout (R2G2), generously donated by Envigo, Spain; and in Hsd-Athymic Nude (Harlan, Barcelona, Spain).

Techniques: Injection, Control, Immunostaining, Expressing